The Rate Of Respiration In Yeast And — страница 5

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1.301?????????????? ???? 2.36??? ?????? 0.373????????????? ??? 30?? 1.477?????????????? ???? 3.31??? ?????? 0.198 ??? 40?? 1.602?????????????? ?? ??7.41??? ?????? 0.870 ??? 42?? 1.623?????????????? ???? 8.55??? ?????? 0.932 ??? 44?? 1.643?????????????? ???? 9.80??? ?????? 0.991 ??? 46?? 1.663?????????????? ???? 10.4??? ?????? 1.017 ??? 48?? 1.681?????????????? ???? 11.2?????????? 1.049??? ??? 50?? 1.699?????????????? ???? 11.6?????????? 1.065? ??? 52?? 1.716?????????????? ???? 12.2?????????? 1.086 ??? 54?? 1.732?????????????? ???? 10.7?????????? 1.029 ??? 56?? 1.748?????????????? ???? 10.2?????????? 1.009 See Attatched DocumentBiology Science 1 – Strand 3: Analysis Summary????????? I found that as the temperature increased, the rate of respiration increased with it. I also

found that the rate of respiration dropped of completely after a certain point, highlighting the denaturisation of the yeast?s enzymes. See Attatched Document ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ????????? ???????????????????????????????????????????? ?????????????????????????????????????????????????? This shows that the temperature See Attatched Document ??????????????????????????????????????????????????????????????????????????????????????????????????????????????? a certain point where respiration ??????????????????????????????????????? ??????????????????????????????????????????????? stops.??????????????????????????????????????????????????????????????? Temp

(?C)To calculate the Q10 gradient of my results so I can gain information about the nature of the reaction, I shall create a graph of my logs given in my Obtaining. From my log graph I can give the optimum temperature for yeast respiration and calculate the Q10 reading for my experiment.I can calculate my Q10 value as shown: ? See Attatched Document See Attatched Document See Attatched Document Conclusion ??????? I have found that as I increased the temperature of the yeast solution, the rate of respiration of the yeast increased to a certain point where, as the temperature rose to a certain level, (in my case about 58?C) the rate of respiration eventually cut off. I have also found that my Q10 value is 1.43. Seeing as the most accurate value for a Q10 reaction is 2 (the rate of

reaction doubling for every 10?C rise) this makes my reaction look a bit inaccurate yet with positive signs of correlation. A Q10 reading as low as 1.43 suggests there were either faults with the method or apparatus or that the reaction was not a true Q10=2 reaction; this reaction should be a typical Q10=2 reaction, so my method or apparatus? probably give the inaccuracies. I will talk further about this in strand iv to suggest reasons. ??????????? My hypothesis and prediction can be backed up with the findings; from looking at my results and graphs you can see the rise and fall of respiration, further displayed by the reaction?s Q10 reading which, although quite a lot less than 2, it still gives the presence of the reaction?s ?sensitivity? (through zymase) to temperature. Thus

my hypothesis and prediction are shown to be present and displayed to a large extent. They are explained due to the theories of enzyme-substrate with lock and key and kinetics. Where these meet is when kinetic theory states that an increase in temperature means more particle collisions between reactants and so a faster rate of reaction; and in enzyme-substrate where the enzyme is sensitive to heat, and about a certain temperature, the active site will begin denaturing, so slowing and eventually stopping the reaction. This will give an area where the rate of respiration drops off and goes to nothing instead of a precise ?cut-off? point. These both apply to my experiment and were described in my planning. ??????????? Biology Science 1 – Strand 4: Evaluation My Method???????????

The experiment went quite well as I was able to obtain sets of recordings and calculate means, rates and logs, and my Q10 value from them.I did not find any results to be anomalous when looking at the results table. This could be explained by the small spread of results at each interval and that the reaction could not be totally accurately controlled with the apparatus used.I think that the method I used, whilst giving results, was also quite sensitive to changes and didn?t allow to tap the full potential of the experiment. I would suggest using equipment which would not allow any biased results or ignore anything that is happening in the solution. I would want to spread out the solution in something like a pert dish to give maximum surface area to help conduct heat and to evenly