The Rate Of Respiration In Yeast And — страница 4

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1????????????????????????????????? 3????????? Care when using thermometer??? ????????????????????????????????????????????????????????????????????????????????????????????? don?t hit hard, or ??????????????????????????????????????????????????????????????????????????????????????????????? ????????????? leave to roll off desk.Biology Science 1 – Strand 2: Obtaining Revised Method??????????? I have made only slight alterations to my proposed method in the planning. I have kept everything the same, except that I shall take multiple readings of smaller intervals around the ?plateau? of my results so as to gain a more accurate understanding of what is happening and where the peak in activity happens. This will also help me to analyse my hypothesis.Variables??????????? ??????????? ?????

??Time taken for solution to decolourise : Dependant Variable ??????????????????????? ???????? Temperature of solution : Independent VariableRange: 20?C – 60?C in 10?C intervals mapping out plateau in 2?C intervals from 42?C – 56?C. Measurements for both variables have been taken and I have calculated the mean after taking three readings at each interval.ResultsSee Attatched Document Time(s)?????????? t1???????????????????? t2???????????????????? t3???????? ??? T(mean)?? Rate (S-1)???? x103 See Attatched Document At 60?C the solution started to decolourise but with the last two, it didn?t fully decolourise showing eventual denaturisation of the yeast?s enzymes. Converting and manipulating data usually proves useful and aids analysis and I have been able to calculate the rates

for my results with my dependant variable using 1/t. As this is inverse, and rates should always be in seconds, the unit I shall use is? S-1. I have made my rate results positive by multiplying them by 103 so making it easier for me to plot and use.To aid the final analysis and to certify precise and reliable results, I decided to map out the top plateau of results at 2?C intervals. The values used cover the rise, peak and fall of the plateau. The results for this are shown below. This will allow me to form an accurate optimum temperature for the respiration of yeast.? had taken all the precautions that I had done previously and used the same method. I will talk about the validity of all my results in my Evaluating.See Attatched Document Temp(?C)?? t1(s)???? t2?????? t3?????

Time(mean)????? Rate (S-1)???????? x103?????? See Attatched Document 42??????? ?????? 125????? 109???? 116????????? 117???????????????? 0.00855????????? ? 8.55 See Attatched Document 44??????? ??????? 96?????? 107????? 105???????? 102???????????????? 0.00980????????? ? 9.80 See Attatched Document 46??????? ??????? 100????? 92?????? 95?????????? 96??????????????????? 0.0104??????????? ? 10.4See Attatched Document ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ??????????????????????????????????????????????? I have ensured that my results are accurate by controlling all the variables stated in my Planning. I also took care when using the equipment so as to retain continuity throughout the experiment.

For this, I checked everything was set up correctly at each reading and prepared my solution in the same way. I did not prepare a ?batch? of solutions as this would have given some more time to acclimatise and more time to react and respire, changing the conditions. When weighing out glucose and yeast on the top pan balance, I checked that the air bubble was always centered and adjusted it accordingly, if left uncentered, this could cause biased results. When measuring out distilled water, I carefully checked that the bottom of the water?s meniscus sat horizontal with the required gradient on the measuring cylinder when looked at from 90? at the side. I also kept the same water in the water bath so as to keep fair the distribution of heat to the test tubes, I mixed this as

well.To further manipulate my results I shall record logs of my results so I can plot this in my analysis. This will also display my results in such a way that will allow me to easily find an optimum temperature for anaerobic respiration in yeast. It will also allow me to calculate the Q10 mean value for my experiment. This would go some way to see the accuracy of my results, but mostly to see whether the reaction is in line with the Q10 theory and regularity of the rate of reaction. I will plot log temp against log rate? to generate my log graph. This is one of many data manipulation methods I shall use in my analysis to find out as much as I can from my data. Here are my log tables including the results taken when plotting out the plateau:See Attatched Document ??? 20??